张祥民老师

科研队伍>>导师>>张祥民老师

个人简历

1983年毕业于河南师范大学获理学学士学位
1986年毕业于大连理工大学获工学硕士学位
1994年毕业于中国科学院大连化学物理研究所获理学博士学位
1986年7月中国科学院大连化学物理研究所国家色谱研究中心工作，任题目组长等职
1994年7月复旦大学做博士后研究工作
1996年晋升副教授，
1999年破格晋升教授
1996-1997年，2004-2005年先后在德国国家环境与健康研究中心、德国Tuebingen大学任高级访问学者、客座教授

教学经历

研究生教学开设《色谱理论基础》《高等仪器分析》《色谱分析》《电泳及其生化应用》等学位专业和选修课；
本科教学参加《现代化学专题》课程的讲授。

学术职务

担任学术期刊Proteomics、《色谱》等编委
担任中国化学会理事、中国色谱学会常务理事、中国化学会色谱专业委员会委员
担任中国蛋白质组学会理事、中国生物化学与分子生物学学会、蛋白质组学专业委员会委员
担任中国分析仪器学会常务理事、上海分析测试协会副理事长、上海市化学化工学会理事等职

张祥民(Xiangmin Zhang) 教授博士生导师
复旦大学生物医学研究院双聘教授(PI)

国家重大基础研究计划（S-973）课题负责人

国家高技术研究计划（863）课题负责人

联系电话：021-55664165
E-MAIL：xmzhang@fudan.edu.cn
联系地址：上海市淞沪路2005号复旦大学江湾校区化学楼4018室

研究方向

主要奖励和荣誉

蛋白质组学新技术新方法
多维色谱分离、生物质谱鉴定新技术
色谱仪器研制及其关键技术
纳米生物材料的制备与应用
中药与天然药物分离与活性研究
环境分析仪器与新技术等

招收培养博士、硕士研究生和博士后60多名。李嫣2011获得"全国百篇优秀博士学位论文"提名奖和上海市"优秀博士学位论文"奖；郝卫强2010获得"国家优秀博士后"奖。2008年获得复旦大学优秀研究生导师称号。
"生物色谱/质谱新技术新方法及其在生物分析中的应用" 2006年获得教育部自然科学一等奖。"基于功能化纳米材料的蛋白质分离分析新方法" 2012年获得教育部自然科学二等奖。

研究成果

发表论文

发展并建立了多维色谱理论、新方法与新技术；提出了阵列色谱多维分离系统方法；发明、研制热膨胀液相色谱仪、便携式气相色谱仪等10多种分析仪器；发现与确认多个生物信号分子及其与癌症、糖尿病等疾病的关系。迄今发表学术论文200余篇，其中Proteomics, Anal. Chem., J Chromatogr. A等SCI文章170余篇，被国际同行引用4000多次（H-index=50）；发表学术著作《现代色谱分析》《色谱理论基础》《蛋白质电泳技术指南》等5部；申请获得26项技术发明专利。

1. Yao, J. Z.; Sun, N. R.; Wang, J. W.; Xie, Y. Q.; Deng, C. H.; Zhang, X. M., Rapid synthesis of titanium(IV)-immobilized magnetic mesoporous silica nanoparticles for endogenous phosphopeptides enrichment. Proteomics 2017, 17 (8), 8.
2. Xu, D. P.; Yan, G. Q.; Gao, M. X.; Deng, C. H.; Zhang, X. M., Highly selective SiO2-NH2@TiO2 hollow microspheres for simultaneous enrichment of phosphopeptides and glycopeptides. Anal. Bioanal. Chem. 2017, 409 (6), 1607-1614.
3. Xu, D. P.; Yan, G. Q.; Gao, M. X.; Deng, C. H.; Zhang, X. M., Selective enrichment of glycopeptides/phosphopeptides using Fe3O4@Au-B(OH)(2)@mTiO(2) core-shell microspheres. Talanta 2017, 166, 154-161.
4. Wang, Y. A.; Wang, J. X.; Gao, M. X.; Zhang, X. M., A novel carbon material with nanopores prepared using a metal-organic framework as precursor for highly selective enrichment of N-linked glycans. Anal. Bioanal. Chem. 2017, 409 (2), 431-438.
5. Shao, X.; Zhang, X. M., Design of five-layer gold nanoparticles self-assembled in a liquid open tubular column for ultrasensitive nano-LC-MS/MS proteomic analysis of 80 living cells. Proteomics 2017, 17 (8), 8.
6. Shao, X.; Chen, M.; Wu, D.; Liu, B. Z.; Zhang, X. M.; Chen, C. Y., Establishment of a two-dimensional liquid chromatography-tandem mass spectrometry system for detection of four tobacco-specific N-nitrosamines. Anal. Methods 2017, 9 (5), 761-767.
7. Li, J.; Wang, J. X.; Ling, Y.; Chen, Z. X.; Gao, M. X.; Zhang, X. M.; Zhou, Y. M., Unprecedented highly efficient capture of glycopeptides by Fe3O4@Mg-MOF-74 core-shell nanoparticles. Chem. Commun. 2017, 53 (28), 4018-4021.
8. Chen, M.; Wang, L. J.; Dong, H. Z.; Shao, X.; Wu, D.; Liu, B. Z.; Zhang, X. N.; Chen, C. Y., Quantitative method for analysis of tobacco-specific N-nitrosamines in mainstream cigarette smoke by using heart-cutting two-dimensional liquid chromatography with tandem mass spectrometry. J. Sep. Sci. 2017, 40 (9), 1920-1927.
9. Zhang, P. M.; Zhang, Y.; Gao, M. X.; Zhang, X. M., Dendrimer-assisted hydrophilic magnetic nanoparticles as sensitive substrates for rapid recognition and enhanced isolation of target tumor cells. Talanta 2016, 161, 925-931.
10. Zhang, P. M.; Gao, M. X.; Zhang, X. M., Functional dendrimer modified ultra-hydrophilic trapping copolymer network towards highly efficient cell capture. Talanta 2016, 153, 366-371.
11. Zhang, L.; Zhang, R.; Gao, M. X.; Zhang, X. M., Facile synthesis of thiol and alkynyl contained SERS reporter molecular and its usage in assembly of polydopamine protected bioorthogonal SERS tag for live cell imaging. Talanta 2016, 158, 315-321.
12. Yao, C.; Wei, C. Y.; Huang, Z.; Lu, Y. Q.; El-Toni, A. M.; Ju, D. W.; Zhang, X. M.; Wang, W. N.; Zhang, F., Phosphorylated Peptide Functionalization of Lanthanide Upconversion Nanoparticles for Tuning Nanomaterial-Cell Interactions. ACS Appl. Mater. Interfaces 2016, 8 (11), 6935-6943.
13. Yang, H. L.; Deng, C. H.; Zhang, X. M., Preparation of Ti4+-immobilized modified silica capillary trapping column for on-line selective enrichment of phosphopeptides. Talanta 2016, 153, 285-294.
14. Xu, D. P.; Gao, M. X.; Deng, C. H.; Zhang, X. M., Ultrasensitive enrichment of phosphopeptides with Ti4+ immobilized SiO2 graphene-like multilayer nanosheets. Analyst 2016, 141 (11), 3421-3427.
15. Xu, D. P.; Gao, M. X.; Deng, C. H.; Zhang, X. M., Synthesis of bifunctional TiO2@SiO2-B(OH)(2)@Fe3O4@TiO2 sandwich-like nanosheets for sequential selective enrichment of phosphopeptides and glycopeptides for mass spectrometric analysis. Anal. Bioanal. Chem. 2016, 408 (20), 5489-5497.
16. Wang, J. X.; Wang, Y. N.; Gao, M. X.; Zhang, X. M.; Yang, P. Y., Versatile metal-organic framework-functionalized magnetic graphene nanoporous composites: As deft matrix for high-effective extraction and purification of the N-linked glycans. Anal. Chim. Acta 2016, 932, 41-48.
17. Wang, J. X.; Wang, Y. A.; Gao, M. X.; Zhang, X. M.; Yang, P. Y., Facile synthesis of hydrophilic polyamidoxime polymers as a novel solid-phase extraction matrix for sequential characterization of glyco- and phosphoproteomes. Anal. Chim. Acta 2016, 907, 69-76.
18. Wang, J. X.; Li, J.; Wang, Y. N.; Gao, M. X.; Zhang, X. M.; Deng, C. H., A novel double-component MOAC honeycomb composite with pollen grains as a template for phosphoproteomics research. Talanta 2016, 154, 141-149.
19. Wang, J. X.; Li, J.; Wang, Y. A.; Gao, M. X.; Zhang, X. M.; Yang, P. Y., Development of Versatile Metal-Organic Framework Functionalized Magnetic Graphene Core-Shell Biocomposite for Highly Specific Recognition of Glycopeptides. ACS Appl. Mater. Interfaces 2016, 8 (41), 27482-27489.
20. Qi, Q.; Yan, G. Q.; Deng, C. H.; Zhang, X. M., A novel protocol for enzymatic digestion based on covalent binding by protein immobilization. Anal. Bioanal. Chem. 2016, 408 (29), 8437-8445.
21. Liu, Y. Y.; Yan, G. Q.; Gao, M. X.; Deng, C. H.; Zhang, X. M., Integrated system for extraction, purification, and digestion of membrane proteins. Anal. Bioanal. Chem. 2016, 408 (13), 3495-3502.
22. Liu, S. S.; Deng, C. H.; Zhang, X. M., Facile synthesis of Cu2+-modified mesoporous silica-coated magnetic graphene composite for enrichment of microcystin-LR followed by mass spectrometry analysis. Talanta 2016, 154, 183-189.
23. Li, L. T.; Zang, W. J.; Zhang, X. M., A high-throughput method for measurement of glycohemoglobin in blood samples utilizing laser-accelerated proteolysis and MALDI-TOF MS. Anal. Bioanal. Chem. 2016, 408 (5), 1507-1513.
24. Li, L. T.; Yan, G. Q.; Zhang, X. M., Laser-assisted proteolysis for accelerating and enhancing protein N-termini analysis. Rapid Commun. Mass Spectrom. 2016, 30 (12), 1398-1402.
25. Li, L. T.; Wu, R. Q.; Yan, G. Q.; Gao, M. X.; Deng, C. H.; Zhang, X. M., A novel method to isolate protein N-terminal peptides from proteome samples using sulfydryl tagging and gold-nanoparticle-based depletion. Anal. Bioanal. Chem. 2016, 408 (2), 441-448.
26. Huang, Z.; Yan, G. Q.; Gao, M. X.; Zhang, X. M., Array-Based Online Two Dimensional Liquid Chromatography System Applied to Effective Depletion of High-Abundance Proteins in Human Plasma. Anal. Chem. 2016, 88 (4), 2440-2445.
27. Zhao, M.; Zhang, X. M.; Deng, C. H., Facile synthesis of hydrophilic magnetic graphene@metal-organic framework for highly selective enrichment of phosphopeptides. RSC Adv. 2015, 5 (45), 35361-35364.
28. Zhao, M.; Zhang, X. M.; Deng, C. H., Rational synthesis of novel recyclable Fe3O4@MOF nanocomposites for enzymatic digestion. Chem. Commun. 2015, 51 (38), 8116-8119.
29. Xiong, Y.; Deng, C. H.; Zhang, X. M.; Yang, P. Y., Designed Synthesis of Aptamer-Immobilized Magnetic Mesoporous Silica/Au Nanocomposites for Highly Selective Enrichment and Detection of Insulin. ACS Appl. Mater. Interfaces 2015, 7 (16), 8451-8456.
30. Wang, Y. A.; Wang, J. X.; Gao, M. X.; Zhang, X. M., An ultra hydrophilic dendrimer-modified magnetic graphene with a polydopamine coating for the selective enrichment of glycopeptides. J. Mat. Chem. B 2015, 3 (44), 8711-8716.
31. Wang, M. Y.; Zhang, X. M.; Deng, C. H., Facile synthesis of magnetic poly(styrene-co-4-vinylbenzene-boronic acid) microspheres for selective enrichment of glycopeptides. Proteomics 2015, 15 (13), 2158-2165.
32. Wang, J. X.; Wang, Y. N.; Gao, M. X.; Zhang, X. M.; Yang, P. Y., Multi layer Hydrophilic Poly(phenol-formaldehyde resin)-Coated Magnetic Graphene for Boronic Acid Immobilization as a Novel Matrix for Glycoproteome Analysis. ACS Appl. Mater. Interfaces 2015, 7 (29), 16011-16017.
33. Wang, J. X.; Gao, M. X.; Yan, G. Q.; Zhang, X. M., An effective and in-situ method based tresyl-functionalized porous polymer material for enrichment and digestion of membrane proteins and its application in extraction tips. Anal. Chim. Acta 2015, 880, 77-83.
34. Sun, N. R.; Zhang, X. M.; Deng, C. H., Designed synthesis of MOF-derived magnetic nanoporous carbon materials for selective enrichment of glycans for glycomics analysis. Nanoscale 2015, 7 (15), 6487-6491.
35. Sun, C. L.; Zhang, R.; Gao, M. X.; Zhang, X. M., A rapid and simple method for efficient capture and accurate discrimination of circulating tumor cells using aptamer conjugated magnetic beads and surface-enhanced Raman scattering imaging. Anal. Bioanal. Chem. 2015, 407 (29), 8883-8892.
36. Sun, C. L.; Zhang, L.; Zhang, R.; Gao, M. X.; Zhang, X. M., Facilely synthesized polydopamine encapsulated surface-enhanced Raman scattering (SERS) probes for multiplex tumor associated cell surface antigen detection using SERS imaging. RSC Adv. 2015, 5 (88), 72369-72372.
37. Liu, Y. Y.; Yan, G. Q.; Gao, M. X.; Deng, C. H.; Zhang, X. M., Membrane protein isolation and identification by covalent binding for proteome research. Proteomics 2015, 15 (22), 3892-3900.
38. Li, L. T.; Yan, G. Q.; Zhang, X. M., Isolation of acetylated and free N-terminal peptides from proteomic samples based on tresyl-functionalized microspheres. Talanta 2015, 144, 122-128.
39. Chen, Q.; Yan, G. Q.; Zhang, X. M., Applying multiple proteases to direct digestion of hundred-scale cell samples for proteome analysis. Rapid Commun. Mass Spectrom. 2015, 29 (15), 1389-1394.
40. Chen, Q.; Yan, G. Q.; Gao, M. X.; Zhang, X. M., Direct digestion of proteins in living cells into peptides for proteomic analysis. Anal. Bioanal. Chem. 2015, 407 (3), 1027-1032.
41. Chen, Q.; Yan, G. Q.; Gao, M. X.; Zhang, X. M., Ultrasensitive Proteome Profiling for 100 Living Cells by Direct Cell Injection, Online Digestion and Nano-LC-MS/MS Analysis. Anal. Chem. 2015, 87 (13), 6674-6680

近五年负责的项目

主持国家重大基础研究（S-973）项目：（2007CB914103）多维、多模式、阵列式蛋白质高效分离新技术研究（2007-2011，课题负责人）、国家高技术研究计划（863）项目：(2012AA020202)蛋白质组富集分离与修饰鉴定的关键技术研究与开发（2012-2015，课题负责人）；承担完成国家863项目（2004BA711A18）"人类健康肝脏蛋白质组表达谱"（2004-2006，子课题负责人）、国家973项目"人类肝脏蛋白质组研究新技术新方法"（2004-2005，骨干）和国家863项目"重大疾病的生理病理的蛋白质组-肝细胞癌变和肝癌转移的蛋白质组研究"（2001-2005，骨干）以及多项国家自然科学基金项目、科学仪器攻关项目等，获得研究经费数千万元。

发明专利

用于高效液相色谱仪的热膨胀连续微流高压梯度泵（ZL 2007 1 0040488.0），高通量蛋白质多维阵列色谱分离系统（ZL 2004 1 0017037.1），一种基于基质辅助激光解吸电离源质谱靶板的蛋白酶解方法（ZL2005 1 0030676.6），一种基于介孔材料涂层的毛细管开管色谱柱及其制备方法（ZL201110053203.3）等26项。

学术交流

在大型国际学术大会或学术论坛上作邀请报告。在第二届世界人类蛋白质组大会（2003年加拿大蒙特利尔）、第33、34、35届国际高效液相色谱大会（2009年日本京都，2010年德国德累斯顿，2011年美国波士顿）、第24和25届国际微尺度分析大会（2009年中国大连，2010年美国圣迭戈）、第36届国际毛细管色谱大会（2012年意大利Riva de Garda）等作特邀报告；并多次参与国家、地区双边学术交流，如中德报告会、中美、中新、海峡两岸、中港分析化学报告会等均作邀请报告。在全国色谱学术报告会、CNHUPO中国蛋白质组学术报告会等全国性学术会议上作邀请报告十多次。